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Ceglu TM

Ceglu TM

CegluTM is a chemically defined scaffold for cell culture developed by SEKISUI CHEMICAL.
It enables uniform coating on a variety of cell culture devices and substrates.

We address the scale-up challenges of culturing induced pluripotent stem cells (iPSCs),
mesenchymal stem cells (MSCs), and more,
thereby contributing to the industrial application of stem cell therapies.

More information

User voice

CegluTM
coating solution

Coming soon

CegluTM
multiwell plate

  • Besides maintenance culture, cells can differentiate into hepatocytes on CegluTM. The detachment issue of epithelial cells has also been improved.

    National Center for Child Health and Development
    Prof. Akutsu

    Besides maintenance culture, cells can differentiate into hepatocytes on CegluTM. The detachment issue of epithelial cells has also been improved.

    Ceglu TM multiwell plate
    Dr. Hidenori Akutsu, National Center for Child Health and Development

    Dr. Akutsu of the National Center for Child Health and Development is working to establish a disease model system for congenital diseases of the liver. When we asked him about his experience using CegluTM, he told us that he was able to greatly reduce the amount of labor required to maintain and culture iPS cells, and that it was also very beneficial in inducing differentiation into hepatocytes.

    • Q.What process did you use CegluTM in your research?

      A.We used it in two processes: one is a culture in which the undifferentiated state is maintained. The other is the differentiation process, the process of inducing differentiation of hepatocytes.

    • Q.How did you evaluate the product after using it?

      A.I am highly satisfied. I would give full marks for undifferentiated maintenance culture. We also obtained the number of cells we wanted.
      Induction of differentiation into hepatocytes, which is also done and easy to use. In previous systems, when cells differentiated and became epithelial cells, they detached after about 2 weeks, but CegluTM has good adhesion and can be maintained and observed for more than 3 weeks.

    • Q.What are the advantages over other scaffold materials?

      A.It is provided in a ready-to-use condition for culture. It has greatly reduced the amount of labor required for experiments. We don't have to do any advance preparation, and we can perform passaging at any time we want.
      In addition, while the adhesion was good, it was not difficult to peel off.

    • Q.What do you expect from CegluTM in the future?

      A.In addition to the 6-well plate, 10cm dish plates are needed. 96-well plates would also be good to have for screening and single-cell subcloning/ sorting purposes.
      It would also be nice to see the addition of scaffold materials specialized for various differentiation inductions.

  • CegluTM facilitates the maintenance culture of iPSCs and shorten the time to obtain results.

    Nagoya Univ.
    Dr. Yamashita

    CegluTM facilitates the maintenance culture of iPSCs and shorten the time to obtain results.

    Ceglu TM multiwell plate
    Dr. Daiki Yamashita,
    Nagoya University.

    Dr. Yamashita of Nagoya University is engaged in research on hematological malignant tumor diseases in children, utilizing iPSCs to create disease models in which mutations are introduced. When we asked Dr. Yamashita how he felt about using CegluTM, he said that he could obtain results more quickly because it reduced the time and effort required to culture iPSCs.

    • Q.What process did you use CegluTM for in your research?

      A.We use it for maintenance culture in an undifferentiated state (We are also trying to induce differentiation using micropatterned plates, which are a under development product.

    • Q.How did you evaluate the product after using CegluTM?

      A.I am very satisfied. I am satisfied with the number of cells obtained and the quality of the cells in terms of maintenance culture.

    • Q.How do you think CegluTM can contribute to the development of your research?

      A.The system has been very helpful in greatly reducing the amount of time and effort required in our busy research activities. I believe that efficient maintenance and culture is now possible, and the time required to obtain research results for differentiation experiments will also reduced.

    • Q.What do you expect from Ceglu in the future?

      A.It would be helpful if we could expand to co-culture experimental systems with other cell types, or if we come up with scaffolds that can carry the necessary elements around the cell mass that is constructed during differentiation, to reduce the process even further.

Ceglu TMfeatures

  • Uniform surface

    Uniform culture surface with minimal lot-to-lot variation.

  • Safe & Stable

    Fully chemically defined with no animal origin component.
    Long-term stability at room temperature.

  • Scalable(coating solution)

    Applied to various cell culture substrates and devices.

  • Ready-to-use(multiwell plate)

    Pre-coated plates are ready-to-use for experiments without any additional preparation.

POINT1Robust long-term culture

CegluTM performs equivalently to Vitronectin, maintaining both pluripotency and the undifferentiated state.

POINT2Uniform culture surface

The uniform culture surface of CegluTMcoated plates has been confirmed through atomic force microscopy (AFM) observation. This ensures the stable culture of stem cells.

Surface observation by AFM

  • CegluTM coated surface
  • Protein scaffold coated surface

POINT3Stable at room temperature

CegluTM can be stored at room temperature without loss of performance for one year.
It is highly stable and ideal for long-term culture.

  • CegluTM storage stability test
    (Before, iPSC)
  • CegluTM storage stability test
    (1 year, iPSC)

POINT4Applied to various substrates and devices

CegluTM can be applied to a wide range of substrates and devices. This ensures a consistent cell culture environment even when the substrate or device is changed.

iPSCs

  • Dish
  • Woven Fabric
  • Microcarrier

MSCs

  • Dish
  • Non-woven Fabric
  • Microcarrier

Examples of iPSC and MSC culture on CegluTM-coated substrates

ProductsProduct

  • For Manufacturers of Cell Culture
    Devices and Substrates

    CegluTM
    coating solution

    CegluTM coating solution can be applied to a variety of substrates and devices. By using this product to coat substrates and devices for your products, you can provide your customers with a uniform cell culture surface for iPSCs, MSCs, and other cell cultures.
    If you require the coating solution, please contact us*.
    *Note that this product is for manufacturers of cell culture equipment and substrates.
    *This product is under development. Please contact us for a sample.

  • For Researchers

    CegluTM
    multiwell plate

    Multiwell plates pre-coated with CegluTM are sterlized and ready to use, eliminating the need for user-applied coating. This feature reduces time and labor in cell culture processes and enhances experimental reproducibility.
    *This product is under development. Please contact us for a sample.

Resources

About CegluTM

Application note

Establishment of iPSCs using CegluTM,
chemically defined scaffold

Conference presentation

If you would like to get a poster for the conference,
please click the link below and fill out the required information.
We will then provide you with the URL for the download page.

Download

Reference

A chemically-defined plastic scaffold for the xeno-free production of human pluripotent stem cells
Scientific Reports. 2022 Feb 15;12(1):2516.
https://doi.org/10.1038/s41598-022-06356-8

Adhesion Characteristics of Human Pancreatic Islets, Duct Epithelial Cells, and Acinar Cells to a Polymer Scaffold
Cell Transplantation. 2022 Sep;31:09636897221120500.
https://doi.org/10.1177/09636897221120500

Improved Production of Induced Pluripotent Stem Cells Using Dot Pattern Culture Plates
Tissue Engineering Part C: Methods. 2023 Sep 1;29(9):410-23.
https://doi.org/10.1089/ten.tec.2023.0068

Size control of induced pluripotent stem cells colonies in two-dimensional culture for differentiation into functional monocyte-like cells
Cytotherapy. 2023 Dec 1;25(12):1338-48.
https://doi.org/10.1016/j.jcyt.2023.08.002

FAQQuestions

Basic information about CegluTM

  • Q1.Are animal-origin components used?

    A.CegluTM is chemically defined materials and NOT contains animal-origin components.

  • Q2.Can I get a Certificate of Analysis (CoA) for my product?

    A.We can provide the CoA, please contact us.

  • Q3.Is there any cytotoxicity?

    A.Cytotoxicity tests was conducted with reference to the US Pharmacopeia (USP87, USP88) test methods, and NO cytotoxicity have been confirmed.

  • Q4.Can I have a sample?

    A.We can provide the sample. Please contact us.

Cell culture with CegluTM

  • Q1.What cell types have you cultured?

    A.We have experiences culturing iPSCs, ESCs, MSCs, fibroblasts and various differentiated cells, and more.

  • Q2.What differentiation experience do you have?

    A.We have experiences with differentiation of iPSCs into trichoderm, hematopoietic stem cells, monocytes, hepatocytes, neurons (dopaminergic), glial cells (astrocytes) and retinal pigment cells, etc.

  • Q3.How long is long-term culture possible?

    A.We have confirmed that iPSCs can be cultured for 10 passages (50-60 days) while maintaining their undifferentiated state.
    For more details, please refer to the following paper.

    Scientific Reports. 2022 Feb 15;12(1):2516.
    https://doi.org/10.1038/s41598-022-06356-8

  • Q4.What cell culture media can be used?

    A.We have extensive experiences culturing iPS cells in various media, including StemFit® AK02N, StemFit® AK03N, StemFlexTM, TeSRTM-E8TM, mTeSRTM1, Essential 8TM and others.

  • Q5.What cell dissociation reagents can be used?

    A.Enzyme-based and chelating reagents can be used for cell dissociation.