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Puel(medium for human iPSCs/ESCs)

Key features

  • High cell proliferation and genomic stability
  • Suitable for 2D/3D, feeder-free/feeder cultures
  • Compliance with standards for biological raw materials

Basic information about Puel

  • Feature 1:
    High cell survival and growth rate

    High growth and survival rates are maintained even in feeder-free culture. Low density cell culture (2x10^3/6well) is also possible, suitable for cell thawing and cell cloning after gene editing.

  • Feature 2:
    Maintain high genomic stability and pluripotency

    Normal karyotype was maintained after 50 passages (n = 6 lines). Pluripotent marker expression was also comparably high.

  • Feature 3:
    2D feeder free, 2D feeder culture, 3D floating culture

    Cells efficiently proliferate in 2D feeder-free and 2D feeder culture. Also suitable for large scale expansion in 3D suspension culture.

  • Feature 4:
    High efficiency iPSC induction

    Fibroblasts, peripheral mononuclear cells, urine-derived cells are efficiently reprogrammed into iPSCs when cultured in Puel. Other iPSC culture media have low reprogramming efficiency.

  • Feature 5:
    Maintain high differentiation potential

    Puel can effectively maintain pluripotency. After long-term culture in Puel, iPSCs can differentiate into neurons, myo-precursor cells, cardiomyocytes, hematopoietic cells, NK cells, T cells and epidermal cells.

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